Figure 6

Quantitative AFM imaging analysis of α-syn fibrils grown for 2 weeks in Cu²⁺ oxidized or Cu²⁺ depleted conditions (using EDTA).

(a) AFM height images of α-syn fibril samples formed in Cu²⁺ oxidized and Cu²⁺ depleted conditions, respectively. The scale bars indicate the length of 10 μm. Magnified (4X) images are shown as insets. (b) Height distributions of the pixels of the fibril particles are shown as histograms, indicating the width of the fibrils formed in Cu²⁺ oxidized and Cu²⁺ depleted conditions, respectively. (c) Length distributions of fibril particles formed in Cu²⁺ oxidized and Cu²⁺ depleted conditions, respectively. The length distributions are show as 1-cumulative distribution functions on a semi-log plot to enable visual comparison for the wide length distributions of the samples. A total of 781 fibrils formed in Cu²⁺ oxidized conditions and 409 fibrils formed in Cu²⁺ depleted conditions were analyzed. 100 μM α-syn shaken for 2 weeks at 37 °C with 100 μM Cu²⁺ or with EDTA (2.5 mM) was diluted to 2 μM for AFM imaging.