Figure 7
Dityrosine cross-links form between fibrils to generate intertwined and interconnected structures.
(a) Schematic showing fibrils connected via dityrosine cross-links, (b) electron micrograph showing dityrosine immunogold labeling of fibrils and (c) AFM image showing interconnected fibrils. α-syn fibrils were formed using 100 uM α-syn incubated with 100 uM Cu2+ in 20 mM HEPES buffer at pH 7.4 with 400 rpm agitation for 30 days incubation.
