Figure 1

The antibacterial effect of TiO₂/Ag⁺-containing porous scaffold materials.

Antimicrobial activity in vitro was evaluated by agar disc-diffusion assay using S. aureus ATCC25923 (A) and E. coli ATCC25922 (B). Uniform discs (1 mm thickness and 7 mm diameter) were placed on to the bacterial inoculated brain heart (BH) agar plates. The plates were incubated under dark conditions for 24 h at 37 °C (a), and the zone of inhibition (ZOI) around the specimen was measured and analyzed (b). The BH agar plates were replaced every 2 days, and ZOI were measured until they disappeared. The vancomycin (VA) and ceftazidime (CAZ) discs (30 μg per tablets) were used as positive controls. Each experiment was repeated five times. Representative results are shown. VA, vancomycin; CAZ, ceftazidime; nHP66, n-HA/PA66 (or nHP66) scaffold material; TA1, 0.22 wt% Ag⁺2.35 wt% TiO₂-nHA/PA66 (or TA1-nHP66) scaffold material; TA2, 0.64 wt% Ag⁺2.35 wt% TiO₂-nHA/PA66 (or TA2-nHP66) scaffold material; A1, 0.22 wt% Ag⁺-nHA/PA66 (or A1-nHP66) scaffold material; A2, 0.64 wt% Ag⁺-nHA/PA66 (or A2-nHP66) scaffold material.