Figure 5
Effect of TrkB antagonist ANA-12 on the alterations in the BDNF-TrkB signaling in the brain regions from susceptible mice after social defeat stress.
(a–f): Forty eight hours after administration of drugs, brain regions from mice were collected. Western blot analysis of BDNF (mature form) and β-actin in the brain regions (PFC, NAc, striatum, CA1, CA3, DG) was performed. The values are expressed as a percentage of that of control mice. Data are shown as mean ± S.E.M. (n = 6–8). *P < 0.05, **P < 0.01, ***P < 0.001 compared to vehicle-treated stress group (one-way ANOVA, followed post hoc LSD test). N.S.: Not significant. (g–l): The ratio of p-TrkB to total TrkB in the brain regions is shown. Representative data of Western blot analyses of p-TrkB, and TrkB in the mouse brain regions. The values are expressed as a percentage of that of control mice. Data are shown as mean ± S.E.M. (n = 6–8). *P < 0.05, **P < 0.01, ***P < 0.001 compared to vehicle-treated stress group (one-way ANOVA, followed post hoc LSD test). N.S.: Not significant.
