Figure 3

HA-MARV replicates robustly in hamsters.

(a–d) Virus titers, expressed as tissue culture infectious dose 50% (TCID₅₀) on a log10 scale, were calculated from the blood (a) the mesenteric lymph nodes (b) the livers (c) and the spleens (d). Mean values for each time point are indicated by the line graph, and individual values for each hamster are indicated by blue (WT-MARV) or red (HA-MARV) dots. n = 3–6. p-values ≤ 0.05 are indicated by a single asterisk (*) and p-values ≤ 0.001 are indicated by two asterisks (**) compared to WT-MARV. (e,f) Immunohistochemistry (IHC) to detect MARV antigen was performed on liver (e) and spleen (f) samples collected 3 or 6 days post-infection with either WT- or HA-MARV. A focus of virus antigen in the liver of a HA-MARV-infected hamster at day 3 is indicated by a white arrowhead. Note that the WT-MARV-infected hamster randomly selected for IHC analysis exhibited no detectable virus titer in the spleen and a very low titer in the liver, which correlates with the absence of antigen detected by IHC in the spleen and the small amount detected in the liver.