Figure 3

NK cell cytotoxicity against HPV+ cervix cancer cells.

(A) The constitutive expression of MICA/B but not ULBPs in HeLa cells, as assessed by flow cytometry. The representative results of three independent experiments are shown. (B) HeLa cells were labeled with CytoTrack green and then co-cultured with PBMC with HNK1/HNK (n = 3) and LNK/LNK (n = 3) for 4 and 16 h. The frequency of cell death is shown. (C) The representative results showing cell death in HeLa cells cultured with PBMCs from an individual with HNK/HNK or LNK/LNK genotype for 16 h, as assessed by flow cytometry. (D) NK cells degranulation (CD3−, CD56+, CD107+) after PBMCs from individuals with HNK/HNK (3) or LNK/LNK (3) genotype were co-cultured with HeLa cells for 16 h and assessed by flow cytometry. The data are shown as the standard deviation ± fold-change (% CD107 without target vs. % CD107 with target).