Figure 3
From: Histone deacetylase inhibitors mediate DNA damage repair in ameliorating hemorrhagic cystitis
DNA methylation analysis of the Ogg1 gene.
(A) Bisulfite sequencing was performed on the Ogg1 gene from promoters region (−800 bp downstream of transcription start site) to exon1 (+336 bp up to transcription start site) from cultured mouse bladder detrusor cells treated with vehicle or acrolein. The CpG islands sequenced fall into regions I, II, III, and IV. The open circles represent the unmethylated cytosine, whereas filled circles represent the methylated cytosine. (B) ChIP analysis targeting DNA methylation binding site within the Ogg1 region III (−41 bp to 103 bp). Dnmt1, Dnmt3A, Dnmt3B, and RNA polymerase II loading onto region III was compared the control and acrolein conditions. Non-immunoprecipitated chromatin was used as total input control. PCR of input DNA shows equivalent starting material for the assay. Normal rabbit IgG antibodies served as the negative control. (C) Three independent ChIP assays on region III of the Ogg1 promoter were analyzed by quantitative PCR for the occupancy of Dnmt1, Dnmt3a, Dnmt3b and Pol II.
