Figure 4
From: Histone deacetylase inhibitors mediate DNA damage repair in ameliorating hemorrhagic cystitis
HDAC inhibitors reverse the Ogg1 mRNA expression in mouse bladder muscle cells.
(A) Bladder muscle cells were transfected with scrambled siRNA or siRNA against mouse Dnmt3b. After 48 h, cells were treated with acrolein for 6 h, and harvested to determine Dnmt3b and Ogg1 mRNA expression by rtPCR. (B) Ogg1 promoter methylation status was determined by methylation specific PCR following acrolein treatment in presence and absence of SAHA. Primers specific for the unmethylated (U) and methylated (M) DNA were used. (C) mRNA expression of Dnmt1 Dnmt3a, Dnmt3b, and Ogg1 were measured in the presence and absence of VPA, SAHA, actinomycin D, and acrolein in cultured bladder muscle cells. The corresponding graph illustrates the mean and minimum/maximum mRNA expression values of the respective band intensity of Dnmt3b and Ogg1. The mRNA expressions were quantitated relative to ß-actin expression (n = 3). One way ANOVA analysis between the control and acrolein treatment are indicated by ##p value < 0.01; #p value < 0.05. Whereas, comparison of the acrolein treatment group and the other groups are indicated by **p value < 0.01; *p value < 0.05.
