Figure 5
From: Histone deacetylase inhibitors mediate DNA damage repair in ameliorating hemorrhagic cystitis
HDAC inhibitors restore Ogg1 expression in mouse bladder.
(A) Bladder inflammation was determined by H&E staining (the scale bar represents 64 μm) and supported by immunohistochemical detection of macrophage by F4/80 staining in the presence or absence of cyclophosphamide and SAHA (arrowheads, the scale bar represents 32 μm). Ogg1 expression was localized by IHC (arrowheads). (B) Corresponding bar diagram is the quantitation of the differential positive staining of F4/80 and Ogg1 (**p value < 0.01; ***p value < 0.001, between groups by one way ANOVA, n = 3). (C) Western blot of two representative bladder tissues from each of the three treatment groups indicate Ogg1 and Dnmt3b expression. The mean quantitations are indicated below each corresponding blot normalized to ß-actin expression.
