Figure 2 | Scientific Reports

Figure 2

From: Biological characteristics comparison of HBV rtA181T mutants with truncated or substituted HBsAg expression in vitro and in vivo model systems

Figure 2

Viral replication after plasmid transient transfection in HepG2 cells.

HepG2 cells were transiently transfected with 10 μg pHBV4.1, pHBV-rtA181T/sW172* and pHBV-rtA181T/sW172L respectively. Cells were collected 3 days after transfection. (A) HBV DNA replication intermediates of HepG2 cells were detected by Southern blotting: 1: pHBV4.1 group 2: pHBV-rtA181T/sW172* group 3: pHBV-rtA181T/sW172L group. (B) Quantitative analysis of HBV DNA replication intermediates. The mean and standard deviations of HBV DNA were also shown and the levels of HBV DNA replication intermediates in pHBV4.1 group 72 hours after transfection was defined as 1.

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