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Figure 3

From: Recombinant Marek’s disease virus type 1 provides full protection against very virulent Marek’s and infectious bursal disease viruses in chickens

Figure 3

Characterization of the recombinant MDVs expressing VP2 gene.

(A) Confirmation of the successful expression of VP2 by indirect immunofluorescence assay. (B) Detection of the expression of VP2 with western blotting. Chicken β-actin was detected as the internal control. (C) Comparison of VP2 expression from different viruses with FACS analysis. Results presented as the mean fluorescence intensity (MFI) of the VP2 positive cells in 10,000 events captured during the procedure. (D) Negative-stain electron microscopy detection of the IBDV subviral particles expressed by rMDV-VP2. Bar length, 40 nm. (E) PCR amplification of the VP2 gene from the recombinant viruses passaged 20 times in CEFs. (F) Detection of VP2 expression from the recombinant viruses passaged 20 times in CEFs with indirect immunofluorescence assay. (G) Comparison of the replication kinetics of the recombinant viruses. Data presented are the means ± standard deviations (S.D.) from three independent experiments. Statistical significance was set at P < 0.05.

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