Figure 3
Non-thermal atmospheric pressure plasma (NTAPP)-exposed adipose tissue-derived stem cells (ASCs) maintain their stem cell properties.
(A) Reverse transcription-polymerase chain reaction (RT-PCR) was performed by using RNA extracted from ASCs. CD44 and CD105 were used as positive markers and CD45 was used as a negative marker for the analysis of ASCs. FABP4 was used as a differentiation marker of ASCs. (B) Expression of the markers of ASCs was analyzed by RT-PCR at 72 h after the first NTAPP exposure and compared to that in unexposed control cells. (C) SA-βGal assay was performed to evaluate senescence in ASCs at 72 h after exposure to NTAPP for a total of 10 times. ASCs treated with 100 μM H2O2 were used as the positive control. Scale bar, 100 μm. Senescent cells were counted, and the values were expressed as percentages. (D) Differentiation of NTAPP-exposed ASCs into adipocytes was induced by incubation for 28 days in adipogenic differentiation medium, and the differentiation of the ASCs was detected using Oil-red O staining. Scale bar, 50 μm. (E) ASCs were evaluated for the expression of an adipocyte marker, FABP4, by using RT-PCR. Random primers were used as negative controls for RT-PCRs.
