Figure 4 | Scientific Reports

Figure 4

From: Surfactin inducing mitochondria-dependent ROS to activate MAPKs, NF-κB and inflammasomes in macrophages for adjuvant activity

Figure 4

Western blotting for analysis of MAPKs in macrophages stimulated with surfactin in vitro and in vivo.

(A) Stimulation with SFN in vitro. SFN (5 μg/ml) obviously increased p38 MAPK and p-p38 MAPK, and slightly increase JNK and p-JNK in macrophages when compared to untreated control. ROS inhibitor could decrease p38 MAPK and JNK expression but not their phosphorylation in macrophages. (B) Stimulation with SFN in vivo. SFN (100 μg/mouse) promoted phosphorylation of ERK, p38 MAPK and JNK in the mouse peritoneal macrophages.

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