Figure 4
From: Amyloid precursor protein modulates Nav1.6 sodium channel currents through a Go-coupled JNK pathway
APP Increases Nav1.6 currents in a Go Protein-dependent manner.
(A–F), Normalized current-voltage relationships of currents in oocytes expressing Nav1.6 α subunits and distinct APP intracellular domains alone (open squares; (A) Full-length APP; (B) AICD; (C) C31; (D) Go protein binding domain, GoBD; (E) Fe65 binding domain, FeBD; (F) VTPEER peptides together with a dominant active mutant of Go protein α subunit (Q205L, closed circles) or a dominant negative mutant of the Go protein α subunit (G203T; closed triangles). (G) Normalized current-voltage relationships of currents in oocytes expressing Nav1.6 α subunits alone (open squares) and together with a dominant active mutant of Go protein α subunit (Q205L, closed circles) or a dominant negative mutant of the Go protein α subunit (G203T; closed triangles). (H) Normalized current-voltage relationships of currents in oocytes expressing Nav1.6 α subunits and APP with (closed circles) or without (open squares) intracellular injection G protein inhibitor NF023. Insert: Representative examples of sodium current responses elicited by depolarizing voltage steps. Error bars represent mean ± S.E.
