Figure 2

Treatment with the ginsenoside Rg1 up-regulates the transrepression and the level of GR via reducing GR acetylation.

HaCaT cells were treated with Dex (1 μM) and/or Rg1 (50 μM) for 1 hour and subsequently exposed to UVB (60 mJ/cm²). Then, the cells were treated with 10 ng/ml TNF-α for 24 hours. (A) Luciferase activity in HaCaT cells transfected with pNF-κB-Luc. (B) GR levels were analysed using western blot assays with total protein isolates. (C) GR mRNA expression were determined using RT-PCR. (D) Acetylation of immunoprecipitated GR was evaluated using immunoblot analysis with anti-acetylated lysine and anti-GR antibodies. Each assay was repeated at least twice. ^ap < 0.05, ^aap < 0.01 vs. vehicle. ^bp < 0.05, ^bbp < 0.01 vs. TNF-α only. ^cp < 0.05, ^ccp < 0.01 vs. TNF-α+UVB.