Figure 2 | Scientific Reports

Figure 2

From: The XBP1 Arm of the Unfolded Protein Response Induces Fibrogenic Activity in Hepatic Stellate Cells Through Autophagy

Figure 2

Coordinate induction of collagen 1-alpha and XBP1 seen following UPR activation from tunicamycin.

Samples were collected after six-hour tunicamycin treatment of LX-2 cells at 0.01, 0.1, 0.25, 0.5, 1.0, 5.0, and 10.0 μg/mL, for (A) RT-PCR and assessed for XBP1 splicing, (B) real time PCR with key fibrogenic genes normalized to the housekeeping gene GAPDH, and expressed as fold change relative to DMSO controls, (C) Western blot of collagen 1-alpha. XBP1 gene expression correlates with that of key fibrogenic genes, and an upregulation of collagen 1-alpha protein is seen at low tunicamycin doses (0.1–1 μg/mL).

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