Table 3 Recovery rates of titin N-fragment in human urine.

From: Establishment of a highly sensitive sandwich ELISA for the N-terminal fragment of titin in urine

Sample

Added Flag-titin-N (pmol/L)

Theoretical Value (pmol/L)

Measured Values (pmol/L)

%

Human Urine (1291.5 pmol/L)

1500.0

2791.5

2823.6

101.5

375.0

1666.5

1717.7

103.1

46.9

1338.4

1330.2

99.4

  1. Purified titin N-fragment proteins (FLAG-Titin-N-CHO, 1500, 375, 46.9 pmol/L each) were added to human urine samples (5-fold diluted) in which 1291.5 pmol/L of titin N-fragment was included. The recovery rates (%) at high, middle and low concentrations were calculated from the measured values (the mean value of duplicate samples) against the theoretical values, respectively. The recovery rates in all concentrations were nearly100% in human urine.
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