Figure 1

Inhibition of TRPC3 channel activity suppresses cardiac fibrosis but not hypertrophy in pressure overloaded mouse hearts.

(a) Representative images of wheat germ agglutinin (WGA) staining for cross-sectional areas (CSA) measurement 6 weeks after TAC (left). Green; WGA, blue; DAPI. Quantitative results are shown in right panel. TRPC3^(+/+)-sham (n = 6), TRPC3^(−/−)-sham (n = 6), TRPC3^(+/+)-TAC (n = 13), TRPC3^(−/−)-TAC (n = 12). (b) Representative images of fibrosis 6 weeks after TAC. (c) Expression levels of hypertrophy-related and fibrosis-related mRNAs in mouse hearts 1 week after TAC. Sham groups (n = 3), TAC groups (n = 5). (d,e) Correlation between fibrosis and hypertrophy 6 weeks after TAC. TRPC3^(+/+)-TAC (n = 13), TRPC3^(−/−)-TAC (n = 12), Vehicle-TAC or pyrazole-3 (Pyr3)-TAC (n = 15). Error bars, s.e.m. *P < 0.05, **P < 0.01.