Figure 5 | Scientific Reports

Figure 5

From: Down-regulation of common NFκB-iNOS pathway by chronic Thalidomide treatment improves Hepatopulmonary Syndrome and Muscle Wasting in rats with Biliary Cirrhosis

Figure 5

Effects of thalidomide treatment on myogenic profiles.

(A) Correlation between muscular 3-nitrotyrosine concentration and M1 macrophages (%); correlation between muscular (B) MyoD and (C) MHC II mRNA expression with 3-nitrotyrosine concentration in anterior tibialis muscle homogenates. (D) Representative immunofluorensence (IF) image and bar graphs of acute effects of thalidomide on fusion index and myotube diameter of C2C12 cells; (E) various mRNA expression in C2C12s’s cell lysates; (F) relative fusion index and (G) myotube diameter in co-culture of CD16+ monocyte collected from different rats with C2C12 cells or co-incubation of supernatant of co-cultured CD16+ monocyte + C2C12 cells with C2C12 cells; (H) ratio of mRNA expressions in co-cultured CD16+ monocyte+C2C12 cells to those in mono-cultured C2C12 cells. #,##p < 0.05, 0.01 vs. S-V; *,**p < 0.05, 0.01 vs. BDL-V; δp < 0.05, 0.01 vs. TNFα groups; †p < 0.05 vs. TNFα+thalidomide (thal) groups.

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