Figure 5
Effect of exogenous metabolites on the susceptibility of A. hydrophila to OXY treatment.
(A,B) The growth curves of A. hydrophila, which were treated with or without 0.6 μg/mL OXY and reversed exogenous metabolites (arginine, lysine). The concentrations of these reagents are in the gradient of 0, 1, 10, and 100 mM. (C to F) The other growth curves of A. hydrophila were treated with or without the final concentration of 0.6 μg/mL OXY and inhibited exogenous metabolites (malic acid in the gradient of 0, 0.3125, 0.625, and 1.25 mM; succinic acid in the gradient of 0, 0.625, 1.25, and 2.5 mM; citric acid in the gradient of 0, 0.078, 0.1563, and 0.3126 mM, and serine in the gradient of 0, 1, 10, and 100 mM). All growth kinetics of treated cells were recorded by absorbance measurements of OD600 nm at 30 °C with the multimode detection platform every hour for 12 hours.
