Figure 5 | Scientific Reports

Figure 5

From: The matrix protein of rabies virus binds to RelAp43 to modulate NF-κB-dependent gene expression related to innate immunity

Figure 5

M-Tha positions 77, 100, 104, 110 are important for viral escape.

HeLa cells were infected by Tha, Th4M or SAD. After 43 hours of infection, the NF-κB pathway was exogenously activated using 10 ng/mL TNF-α during 5 h (black bars) or left untreated (grey bars) and total RNA was extracted. The expression level of the following genes: IFNβ (A), TNF (B), IL8 (C), CXCL2 (D) was studied by RT-qPCR analysis. Significant results (p < 0.05) compared to the untreated (*) or TNF treated (**) mock and to the untreated (#) or TNF treated (##) Th4M infected cells are annoted. The levels of gene expression were normalized according to the level of GAPDH reporter gene in non-infected and untreated cells.

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