Figure 6 | Scientific Reports

Figure 6

From: The matrix protein of rabies virus binds to RelAp43 to modulate NF-κB-dependent gene expression related to innate immunity

Figure 6

RelAp43 is involved in the modulation of genes related to innate immunity.

HeLa cells were infected by Tha, Th4M or SAD virus and treated with a siRNA control (siCTRL) or a siRNA directed against RelAp43 (siRelAp43). After 48 hours of infection, total RNA was extracted. The efficiency of siRNA treatment was evaluated by the measure of RelAp43 mRNA expression by RT-qPCR (A). The expression level of the following genes: IFNβ (B), TNF (C), IL8 (D), CXCL2 (E) was studied by RT-qPCR analysis. Significant results (p < 0.05) compared to the siCTRL of mock (*) and Tha () infected cells are annoted. (#) indicated significant results between the siCTRL treated sample and the siRelAp43 respective one (p < 0.05). The levels of gene expression were normalized according to the level of GAPDH reporter gene in non-infected and untreated cells.

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