Figure 2: MiR-218 expression and its regulation of the Wnt signaling pathway assessed by the Western blotting and qRT-PCR. | Scientific Reports

Figure 2: MiR-218 expression and its regulation of the Wnt signaling pathway assessed by the Western blotting and qRT-PCR.

From: MiR-218 Induces Neuronal Differentiation of ASCs in a Temporally Sequential Manner with Fibroblast Growth Factor by Regulation of the Wnt Signaling Pathway

Figure 2

(a) In the +RA group (RA-treated group), based on the database prediction (TargetScan version 6.2 database) and the KEGG analysis, the genes involved in the Wnt signaling pathway (frame fill with green) and several key genes (red pentacles) in the Wnt signaling pathway. (b) Diagram showing the link between the key miRNAs and its target genes which are involved in or targeted on the regulation of Wnt signaling pathway. The genes included in Wnt signaling pathway (red pentacles) and regulated Wnt signaling (blue pentacles) are targeted by miRNAs (blue circles) and the key miRNAs (miR-218, miR-29a and miR-200 et al.) are summarized according the Z-test ranking results. MiR-218 (Z-test = 42.3) is found to be in the front rank. (c) Relative miR-218 expression for the control group (RA-non-treated cells), RA group (RA-treated for 15 days) and anti-miR-218/RA group (ASCs are transfected by anti-miR-218 plasmids for 48 h and RA treated for 15 days) are determined by qRT-PCR (**p < 0.01, n = 8). (d) Western blotting analysis of OCT4, SOX2 and βIII-TUBULIN expression in the control group, RA group and anti-miR-218/RA group and GAPDH is used as control. (e) Protein expression levels, quantified by determining the gray value (n = 3).

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