Figure 3: Expression of miR-218 and of components of the Wnt signaling pathway on ASCs neuronal differentiation, monitored by Western Blotting and qRT-PCR. | Scientific Reports

Figure 3: Expression of miR-218 and of components of the Wnt signaling pathway on ASCs neuronal differentiation, monitored by Western Blotting and qRT-PCR.

From: MiR-218 Induces Neuronal Differentiation of ASCs in a Temporally Sequential Manner with Fibroblast Growth Factor by Regulation of the Wnt Signaling Pathway

Figure 3

(a) The relative miR-218 expression levels for ASCs transfected with control plasmid (miR-NC group), miR-218 plasmid (miR-218 group) and anti-miR-218 plasmid (anti-miR-218 group) after 48 h are detected by qRT-PCR (**p < 0.01, n = 4). (b) The qRT-PCR results of some miR-218 target (Robo1, Robo2, Lamb3, Sfrp2 and Dkk2) mRNAs expression, detected in miR-NC group, miR-218 group and anti-miR-218 group. (c) Western blotting results of Wnt signaling pathway markers (FZD and p-FZD in the cytoplasm and β-CATENIN in the nuclear) in the miR-NC group, miR-218 group and anti-miR-218 group and GAPDH is used as control. (d) Proteins expression levels, quantified by determining the gray value (n = 2). (e) The qRT-PCR results of Oct4, Sox2, βIII-Tubulin, Map2 and Nestin mRNA expression levels in the RA-treated group (RA group) and three plasmid treated groups (miR-NC group, miR-218 group and anti-miR-218 group). (f) Western blotting results of βIII-TUBULIN, MAP2 and NESTIN in the four groups (RA group, miR-NC group, miR-218 group and anti-miR-218 group) and GAPDH is used as control. (g) Protein expression level, quantified by determining the gray value (n = 2).

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