Figure 3 | Scientific Reports

Figure 3

From: Antibodies from multiple sclerosis patients preferentially recognize hyperglucosylated adhesin of non-typeable Haemophilus influenzae

Figure 3

Immunoaffinity purification of antibodies from one representative NBD and one MS serum.

(a) Schematic process of antibody fractionation. (b) SP-ELISA of antibody fractions obtained from two sequential sepharose columns bearing the immobilized non-glucosylated I and hyperglucosylated I(Glc). Antigen I designated as solid bars and antigen I(Glc) designated as gray bars. In the case of NBD serum, flow through 1 (FT1) and eluted fraction 2 (Elu2) were collected. In the case of MS serum, flow through 1 (FT1), eluted fraction 2 (Elu2), flow through 3 (FT3), eluted fraction 4 (Elu4) were collected. Each point is mean ± s.d. for n = 3 independent experiments. The ELISA absorbance in serum can be explained considering that IgG concentration in the whole sera (10 mg/ml) is 300 times higher than in eluted fractions (0.03 mg/ml).

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