Figure 3 | Scientific Reports

Figure 3

From: Induction of a Cellular DNA Damage Response by Porcine Circovirus Type 2 Facilitates Viral Replication and Mediates Apoptotic Responses

Figure 3

PCV2-induced DDR requires viral replication.

(A) PK15 monolayer cells were infected with wild-type or UV-irradiated PCV2 at an MOI of 1 TCID50. (B) Images of PCV2 VLPs. Bar, 100 nm. (C) PK15 monolayer cells were inoculated with PCV2 virus-like particles (10 VLPs per cell). Cell lysates at 48 h post infection or inoculation were harvested and subjected to SDS-PAGE followed by immunoblotting with antibodies to H2AX, RPA32, ATM, ATR, and DNA-PK phosphorylated forms. β-actin was acted as the loading control. p-, phosphorylated.

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