Figure 5
From: Mice lacking WRB reveal differential biogenesis requirements of tail-anchored proteins in vivo
Stx5 is degraded by a FAM134B-dependent autophagy mechanism.
(a) Control-silenced HeLa cells or knockdown cells for WRB or TRC40 were co-transfected with a control siRNA or with a FAM134B-specific siRNA. Cellular lysates were analyzed by Western blot for the indicated proteins. Graphs in (b,c) show quantification of the expression levels of Stx5 and Stx8 respectively. Bars represent average +/−s.e.m. (n = 6, **p-value < 0.01). (d) Control-silenced HeLa cells or knockdown cells for WRB or TRC40 were co-transfected with a control siRNA or with a p62-specific siRNA. Cellular lysates were analyzed by Western blot for the indicated proteins. Graphs in e,f show quantification of the steady-state levels of Stx5 and Stx8 respectively. Bars represent average +/−s.e.m. (n = 4, variations not significant).
