Figure 3
QS-21-bodipy rapidly accumulates in the draining lymph node where it colocalises with CD11b+ CD169+ macrophages of the subcapsular sinus.
(A) Mice were injected i.m. with liposomes containing bodipy-labelled QS-21. The draining lymph nodes were recovered 30 min and 3 h post injection and stained with anti-CD11b, anti-CD169 and anti-B220 antibodies and analysed by confocal microscopy. (B) Flow cytometry analysis of subcapsular sinus (CD169+ F4/80−) and medullary sinus (CD169+ F4/80+) macrophages in mice 24 h following PBS or QS-21 injection. (C) Quantification of the loss of LN-resident macrophages detected by flow cytometry. Results from two independent experiments. Each point represents one mouse. Statistical significance was determined by a non-parametric Mann-Whitney test.
