Figure 5
Batf3-dependent dendritic cells regulate QS-21-mediated T-cell responses.
Mice were immunised as in 1 A and adaptive responses were measured at day 21. (A) Flow cytometry analysis of cytokine production by HBs-specific CD4 T cells in WT and Batf3−/− mice. (B) polyfunctionality index of CD4 T cells. (C) Flow cytometry analysis of cytokine production by splenic HBs-specific CD8 T cells and of the frequency of circulating OVA-specific CD8 T cells (Ag: n = 1–2, QS-21: n = 8). (D) Anti-HBs IgG1 and IgG2c titres in the serum measured by ELISA (Ag: n = 1–2, QS-21: n = 8). Each point represents one mouse and the horizontal bar represents the geometric mean. Statistical significance was determined by a non-parametric Mann-Whitney test.
