Figure 2
IFG upregulates the expression of CD40, CD80, CD86, and HLA-DR on the Mtb infected macrophages.
[A–D] Macrophages; [E,F] Human PBMCs were infected with Mtb for 4 h and subsequently stimulated with IFG (64 ng/ml) for 24 h. Later, expression of CD40, CD80, CD86, and HLA-DR was evaluated by flow cytometry on the [A–D] macrophages; [E,F] CD11b gated PBMCs macrophages. The flow cytometry data (iMFI) represented through bar diagrams as mean ± SEM are representative of two independent experiments. UI: uninfected macrophages; UI + IFG: uninfected macrophages stimulated with IFG; US: Mtb infected macrophages; Infected + IFG: Mtb infected and IFG stimulated macrophages. *p ≤ 0.0285, **p < 0.003, ***p < 0.0004, ****p < 0.0001.
