Figure 1 | Scientific Reports

Figure 1

From: Leptospira surface adhesin (Lsa21) induces Toll like receptor 2 and 4 mediated inflammatory responses in macrophages

Figure 1

Testing of TLR activity of rLsa21.

rLsa21 was screened for TLR2 activity on HEK293 cell lines transfected with TLR2 and NF-κB driven reporter plasmids by Luciferase assay (A) and induction of IL-8 (B) after stimulation with the protein and pretreatment with PK or blocking with TLR2 antibodies as described in material and methods. UI indicates uninduced or unstimulated cells, GST- indicates Glutathione S-transferase protein (2 μg/ml) PAM indicates PAM3CSK (20 ng/ml), PK is Proteinase K, PB is Polymixin B (10 μg/ml) and TLR is Toll like receptors.

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