Figure 3 | Scientific Reports

Figure 3

From: Leptospira surface adhesin (Lsa21) induces Toll like receptor 2 and 4 mediated inflammatory responses in macrophages

Figure 3

rLsa21 induced cytokine secretion in MyD88−/− and TRIF−/− mouse macrophages.

WT, MyD88−/− and TRIF−/− peritoneal macrophages cell lines were treated with rLsa21 (2 μg/ml), LPS (500 ng/ml) or PAM3CSK (20 ng/ml) and levels of IL-6 (A) and TNF-α (B) in the supernatants were measured with ELISA. Significant differences were calculated between control and treatment groups using the Student’s t-test (*Indicates P < 0.05). UI indicates uninduced or unstimulated cells, PAM indicates PAM3CSK (20 ng/ml) and LPS is E. coli Lipopolysaccharide (500 ng/ml).

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