Figure 6
Analysis of rLsa21 induced expression of surface markers on the mouse macrophages.
RAW264.7 cells were incubated with LPS (500 ng/ml) or PAM3CSK (20 ng/ml) or rLsa21 (2 μg/ml) for 24 hrs at 37 °C/5% CO2 Cells were stained with isotype control Ab or PE-CY5-conjugated anti-MHC-II. FITC-conjugated anti-CD86 or R-phycoerythrin-conjugated anti-CD80 as described in materials and methods and then analyzed by Flow cytometry. UI indicates uninduced or unstimulated cells, PAM indicates PAM3CSK (20 ng/ml) and LPS is E. coli Lipopolysaccharide (500 ng/ml).
