Figure 7
rLsa21 induced gene expression profiling in mouse macrophages.
WT, TLR2−/−, TLR4−/− and TLR2−/−/4−/− mouse macrophage cell lines were treated with rLsa21 (2 μg/ml), LPS (500 ng/ml) or Pam3CSK4 (20 ng/ml). After treatment at various time points (4 hrs, 24 hrs, 48 hrs) cells were recovered in 500 μl of TRIzol, RNA was purified and converted to cDNA. RT-PCR was performed in 96 well microtiter plates on an ABI 7500 sequence detection system as described in material and methods. The experimental data were presented as fold changes of gene expression of stimulated cells at various time points relative to control. mRNA levels of the analyzed genes were normalized to the amount of β-actin present in each sample. UI indicates uninduced or unstimulated cells, PAM indicates PAM3CSK (20 ng/ml) and LPS is E. coli Lipopolysaccharide (500 ng/ml).
