Figure 1
Live-imaging system at the ISS.
(a) Left photo shows a top view of the medaka chamber. Scale bar = 1 cm. Right photo shows a whole image of a medaka larva that is seen in the enlarged view of the black-squared area in the medaka chamber. Scale bar = 1 mm. (b) Cartoon showing a lateral view of a medaka larva embedded in the Mebiol gel. The fish at stage 39 were observed via fluorescence microscopy from the bottom side. Scale bar = 1 mm. (c) Order of observation via remote operation. Three steps were required to get accurate location of medaka larvae. Step 1: At first, XY coordinate was created by using a 5x objective lens. The red arrows show the direction of capture on the XY plane. The observed objects were selected. Step 2: XY coordinates were corrected, and the Z coordinate was created by using the 10x objective lens. The blue crosses show the observed objects whose ventral side was oriented toward the glass plate for observation via an objective lens; and red arrows, the direction of capturing Z-axis with a width of 1000 μm. Step 3: Clear fluorescent images were captured by using a 20x objective lens with a width of 500 μm. The steps are summarized in the table at the bottom of Fig. 1.
