Figure 7: Expression of GmCYP1 and isoflavonoid biosynthetic genes in response to stress.
From: Soybean cyclophilin GmCYP1 interacts with an isoflavonoid regulator GmMYB176
Etiolated soybean hypocotyls were treated with either 1 mM AgNO3 or water (control) for 24, 48 or 72 h, and tissues were used to evaluate (a) GmCYP1 (b) isoflavonoid biosynthetic gene (GmCHS8, GmCHI1B1, GmIFS2, GmPT) transcript accumulation using quantitative RT-PCR. Error bars indicate SEM of two biological replicates and three technical replicates for each biological replicate. The data were normalized against the SUBI-3 gene for GmCYP1 and CON4 gene for isoflavonoid genes. Asterisk (*) indicate significant difference between the samples using Student’s t-test.
