Figure 6 | Scientific Reports

Figure 6

From: PRKAR1A is a functional tumor suppressor inhibiting ERK/Snail/E-cadherin pathway in lung adenocarcinoma

Figure 6

PRKAR1A regulated E-cadherin expression through ERK/Snail/E-cadherin signaling.

(A–C) PRKAR1A inhibited the expression of Snail1. (A and B) The correlation between PRKAR1A and Snail1 or Snail2 was analyzed by Pearson’s correlation. The mRNA data was retrieved from TCGA by cBioPortal, n = 522. (C and D) qRT-PCR showed that PRKAR1A affected the expression of Snail1. n = 3, bar: SD. (D) Phospho-ERK1/2 detected in A549 and H1299 cells either downregulated or upregulated the PRKAR1A gene. The bands of p-ERK1/2 were normalized to ERK protein. (E) A549 cells with stable PRKAR1A knockdown were treated by ERK inhibitor U0126 for 24 h. WB was assessed detect the expression of E-cadherin, p-ERK1/2, and Snai1 (encoded by Snail). p–ERK1/2 band normalized by ERK, E-cadherin, and Snai1 bands normalized to GAPDH.

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