Figure 1 | Scientific Reports

Figure 1

From: Cross-protective efficacy of dendritic cells targeting conserved influenza virus antigen expressed by Lactobacillus plantarum

Figure 1

Plasmid construction and protein synthesis.

(a) The pSIP409-NP-M1-DCpep was generated as described in the Methods. NP: nucleo-protein gene; M1: matrix protein gene; DCpep: dendritic cell-targeting peptide; ermL: erythromycin-resistance marker; 256rep: replication origin for Lactobacillus; PorfX and PsppIP: inducible promoters; sppR and sppK: response regulator and histidine protein kinase, respectively. (b) Western blotting of the synthesized proteins. All cultures were induced with SppIP for 8 h and then subjected to an immunological assay using an anti-NP prime antibody. Lane 1: negative control, NC8(pSIP-409); lane 2: NC8(pSIP409-NP-M1-Ctrlpep), lane 3: NC8(pSIP409-NP-M1-DCpep); and lane 4: Purified NP protein.

Back to article page