Figure 3 | Scientific Reports

Figure 3

From: Cross-protective efficacy of dendritic cells targeting conserved influenza virus antigen expressed by Lactobacillus plantarum

Figure 3

Local immune responses induced by recombinant vaccines.

C57BL/6 mice were orally administered NC8 strains harbouring each plasmid as indicated on days 1, 2 and 3, and were then boosted on days 28, 29 and 30. (a) Fourteen days after booster immunization, the number of B220+ IgA+ cells in PP was measured by flow cytometry (n = 5/group). (b) The total sIgA in faeces was measured by ELISA (n = 8~10/group) on days 14 and 44 after primer immunization. (c) The specific sIgA titres in the faecces and BALF were analysed 44 days after the primer vaccination (n = 5/group). The results are presented as the means ± S.E.M and were analysed by using a one-way ANOVA, assuming a Gaussian distribution followed by Dunnett’s post-test and are expressed relative to the values for PBS, NC8-pSIP409, NC8-pSIP409-NP-M1-Ctrlpep and to the H9N2 inactivated vaccine (*P < 0.05, **P < 0.01, and ***P < 0.001). The data shown represent one of the three experiments with equivalent results.

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