Figure 8
Vaccination with recombinant L.plantarum strains contributes to the proliferation of antigen-specific CD4 T cells and CD8 T cells in the dLN, spleens and MLN.
(a) Thirty days after booster vaccination, the dLN, spleens and MLN were collected from each group and stained with CFSE. A total of 5 × 105 cells were incubated with the NP and M1 peptides for four days, and flow cytometry was performed to detect specific proliferation. (b) The histogram indicates the CD4:CD8 ratio in the dLN, spleens and MLN of the NC8-pSIP409-NP-M1-Ctrlpep group and the NC8-pSIP409-NP-M1-DCpep group. The results are presented as the mean ± SEM of triplicate tests (n = 5 mice per group) and were analysed by using an unpaired t-test. The data are expressed relative to NC8-pSIP409-NP-M1-Ctrlpep (*P < 0.05). The data shown represent one of three experiments with equivalent results.
