Figure 9
Protective effect of transferred NC8-pSIP409NP-DCpep-primed CD8+ T cells in NOD/SCID mice.
(a) CD8+ T cells were isolated from the spleens and MLN of mice immunized with the different strains (as indicated) 14 days after booster vaccination and were then adoptively transferred to NOD/SCID mice by i.v. (tail vein) injection. After 24 h, the NOD/SCID mice were infected with 0.5 × LD50 of mouse-adapted H9N2 AIVs and subsequently monitored daily for four weeks after infection. (b) The weight loss of the mice infected with mouse-adapted H9N2 AIVs was measured daily until day 28. (c) The survival rates (percentages) of the NOD/SCID mice were monitored for four weeks after infection. The results are presented as the means ± SEM of triplicate tests (n = 5 mice per group). The weight loss data were analysed by using a one-way ANOVA, assuming a Gaussian distribution, followed by Dunnett’s post-test. The mortality data were analysed by using the Log-rank (Mantel-Cox) test and are expressed relative to the NC8-pSIP409 and NC8-pSIP409-NP-M1-Ctrlpep groups (*P < 0.05). The data shown represent one of the three experiments with equivalent results.
