Figure 1: Inhibition of PlAMV propagation in ncbp mutants. | Scientific Reports

Figure 1: Inhibition of PlAMV propagation in ncbp mutants.

From: Deficiency of the eIF4E isoform nCBP limits the cell-to-cell movement of a plant virus encoding triple-gene-block proteins in Arabidopsis thaliana

Figure 1

(a) Green fluorescence emission of PlAMV-GFP in inoculated leaves of A. thaliana mutant lines of eIF4E family genes. Mutant lines and Col-0 were inoculated mechanically with PlAMV-GFP-infected leaf extracts and photos were taken under a fluorescence microscope at 4 days post inoculation (dpi). Representative images are shown. (b) PlAMV RNA accumulation in mutant lines measured by quantitative RT-PCR. Total RNA extracted from inoculated leaves in (a) at 4 dpi was subjected to quantitative RT-PCR using RdRp-specific primers to detect viral genomic RNA. The accumulation of PlAMV-GFP RNA normalized relative to that of actin mRNA is presented in each sample. The mean level of viral RNA in Col-0 was used as the standard (1.0). Error bars represent standard errors of 12 measurements from three independent experiments. Double asterisk indicates a significant difference compared with Col-0 (two-tailed Dunnett’s test, double asterisk; P < 0.01, triple asterisk; P < 0.001). (c) Fluorescence images of PlAMV-GFP in systemic plants. PlAMV-GFP was inoculated mechanically onto ncbp-1 and Col-0 and photos of fluorescence (upper and middle panels) and bright-field (lower panels) images of systemic plants were taken at 21 dpi. Bars, 3.5 cm. Middle panels are magnified images of upper panels. (d) Detection of PlAMV-GFP by RT-PCR in upper leaves. Upper leaf samples in (c) at 21 dpi were analyzed by RT-PCR using CP-specific primers with the actin gene as an internal control. Cropped gel images are shown and full-length gel images are included in Supplementary Figure S7. Experiments were replicated three times.

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