Figure 2: Functional validation of nCBP by transgenic complementation. | Scientific Reports

Figure 2: Functional validation of nCBP by transgenic complementation.

From: Deficiency of the eIF4E isoform nCBP limits the cell-to-cell movement of a plant virus encoding triple-gene-block proteins in Arabidopsis thaliana

Figure 2

(a) Accumulation of nCBP protein in A. thaliana transformants. Total protein was extracted from rosette leaves of Col-0, the ncbp-1 mutant, and three nCBP-complemented transgenic lines, and analyzed by immunoblotting, using antisera recognizing nCBP. The cropped image is shown and the full-length blot is included in Supplementary Figure S8. Experiments were replicated twice. (b) Green fluorescence emission from PlAMV-GFP-inoculated leaves of A. thaliana transformants. Transformant lines as well as Col-0 and ncbp-1 mutants were inoculated mechanically with PlAMV-GFP and photos were taken at 4 dpi. Representative images are shown. (c) PlAMV RNA accumulation in inoculated leaves of A. thaliana transformants. Total RNAs extracted from inoculated leaves of corresponding plants in (b) at 4 dpi were analyzed based on quantitative RT-PCR using RdRp-specific primers. PlAMV RNA accumulation was normalized relative to the actin mRNA value in each sample. The mean level of viral RNA in Col-0 was used as the standard (1.0). Error bars represent standard errors of at least 10 measurements from three independent experiments and the asterisk indicates a significant difference compared with Col-0 (two-tailed Dunnett’s test, asterisk; P < 0.05).

Back to article page