Figure 6: nCBP is required for the efficient accumulation of viruses in Alphaflexiviridae and Betaflexiviridae. | Scientific Reports

Figure 6: nCBP is required for the efficient accumulation of viruses in Alphaflexiviridae and Betaflexiviridae.

From: Deficiency of the eIF4E isoform nCBP limits the cell-to-cell movement of a plant virus encoding triple-gene-block proteins in Arabidopsis thaliana

Figure 6

The ncbp-1 mutant, Col-0, and the nCBP-complemented line (3 F) were inoculated mechanically with AltMV (a), CymMV (b), LoLV (c), PVM (d), TuMV (e), and YoMV (f), and total RNA was extracted from inoculated leaves at 4 dpi. Virus accumulation was analyzed by quantitative RT-PCR using virus-specific primers. The accumulation of viral RNA was normalized relative to actin mRNA in each sample. The mean level of viral RNA in Col-0 was used as the standard (1.0). Error bars represent standard errors of 12 measurements from three independent experiments and asterisk and double asterisks indicate significant differences compared with Col-0 (one-tailed Student’s t-test, asterisk; P < 0.05, double asterisk; P < 0.01).

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