Table 1 The Cry1Ac LC50 and 95% confidence intervals (CI) for H. zea neonates.

From: Nutrition affects insect susceptibility to Bt toxins

Diet

Diet P:C Ratio

Diet total macronutrient content (P + C)

LC50

95% CI

Commercial Diet (CD)

0.43

62%

0.107

0.04–0.32

Modified Commercial Diet #1 (MCD1)

1.6

62%

8.185

1.82–37.15

Modified Commercial Diet #2 (MCD2)

1.6

46%

10.304

2.44–43.65

  1. Three diets were tested. One was a carbohydrate-biased commercial diet (CD) commonly used for rearing caterpillars, and in Bt-resistance bioassays. The two other diets were modified versions of the CD. In both cases the modified commercial diets (MCD) had protein-carbohydrate ratios that matched the self-selected intake target of H. zea (1.6:1 P:C). The first of these (MCD1) had a total macronutrient (P + C) content equal to the CD. The other (MCD2) had a lower total macronutrient content, which is more in line with total macronutrient content found in plant vegetative tissues.