Table 1 Characteristics of M. sympodialis cultures and isolated MalaEx after 48 h culture at different pH values.

From: Identification of small RNAs in extracellular vesicles from the commensal yeast Malassezia sympodialis

Batcha

M. sympodialis

MalaEx

pH at start at harvest

Total cell number at harvest (×109)

Mean vesicle sizeb (nm)

Total no. of released vesiclesb (×1012)

Protein concentrationc (mg/ml)

A (n = 5)

5.5 ± 0.03

5.3 ± 0.01

201 ± 66

193.9 ± 9.9

249 ± 43.7

0.80 ± 0.26

B (n = 5)

6.1 ± 0.02

5.9 ± 0.04

261 ± 54

213.2 ± 12.0

164 ± 15

1.14 ± 0.52

p-valued

  

0.142

0.096

0.055

0.226

  1. aAll batches had a cell concentration of 2 × 106cells/ml in 300 ml mDixon broth from start. bThe analysis was done using the LM 10-platform with sCMOS camera from NanoSight. cThe protein concentration was measured using a DC protein assay from BioRad. dP values were calculated using a paired t-test. The values represent mean ± SD of five independent pairwise cultures.
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