Figure 2: Soluble OX40L-JAG1 can cause selective Treg proliferation independent of TCR stimulation.

(A) CD4+ T-cells were treated with IL-2 (Control), OX40L-JAG1-IL-2 and anti-CD3/CD28-IL-2 for 3 days. Extent of CD4+Foxp3- (Teff) and CD4+Foxp3+ (Treg) cell proliferation was analyzed by flow cytometry. (B) From the above experiments, percentages of CD25, CD44 and CD69 expressing Teff (Grey) and Treg (Black) cells were gated and indicated as numerical. (C,D) Bar graph showing percentages of Teff cells and Treg cells expressing CD25, CD44 and CD69. Values are expressed as Mean ± SEM (n = 3; *p < 0.05, **p < 0.01, ***p < 0.001 Vs control).