Figure 1

Alignment of (a) the proline-rich segments in U24, MBP, and Nogo-A, and (b) the WW domains used in this study. (a) The numbering of MBP is based on the human 18.5 kDa classic MBP (Note that in ref. 14, the murine numbering 90–104 was used). The identical segment between U24-6A and MBP, PRTPPPS, is marked with yellow shading. The PxxP motif is marked by the solid lined box. U24-7, pU24-6A and Nogo-A are aligned with respect to U24-6A and their common PY motifs are marked with a dashed box. The phospho-threonine in pU24-6A is marked as pT. The positively and negatively charged residues are shown in blue and red, respectively. (b) The alignment of WW domains from rNedd4, hNedd4 and hNedd4L. The conserved tryptophans are highlighted in bold. The numbering of the domains is given in the subscript next to the name.