Figure 3: ITC result of three U24 peptide titrated into Nedd4-WW domains. | Scientific Reports

Figure 3: ITC result of three U24 peptide titrated into Nedd4-WW domains.

From: U24 from Roseolovirus interacts strongly with Nedd4 WW Domains

Figure 3

Normalized heats and fits are shown for U24-6A peptide in blue, pU24-6A in green and U24-7 peptide in red. The peptides are titrated into a solutions of (a) rNedd4-WW3/4 domain, (b) hNedd4L-WW3* domain. The fitted ΔH of ITC experiments done in different buffers were plotted as bar graphs in (c) U24 peptides were titrated into rNedd4-WW3/4 domain or (d) titrated into hNedd4L-WW3* domain. All titrations are done at 25 °C. Error bars indicate ± one standard deviation, obtained from three repeats. n.s., nonsignificant; *P ≤ 0.037, **P ≤ 0.0088, ***P ≤ 0.0001 by Student’s t test.

Back to article page