Table 1 Parameters obtained from fitting the ITC data for binding of U24 peptides to Nedd4-WW domains at 25 °C.

From: U24 from Roseolovirus interacts strongly with Nedd4 WW Domains

 

U24-6A

pU24-6A

U24-7

Nogo-A

KD (μM)

ΔH (kJ/mol)

KD (μM)

ΔH (kJ/mol)

KD (μM)

ΔH (kJ/mol)

KD (μM)

ΔH (kJ/mol)

rNedd4-WW2

155 ± 12

−54 ± 5

  

87 ± 5

−76 ± 3

  

rNedd4-WW3/4

43 ± 2

−46 ± 2

13.5 ± 0.4

−54 ± 1

21.0 ± 0.5

−63.5 ± 0.5

  

hNedd4-WW3*

9.7 ± 0.2

−53.0 ± 0.1

  

1.98 ± 0.04

−70.1 ± 0.2

  

hNedd4L-WW3*

6.3 ± 0.3

−55.3 ± 0.3

0.76 ± 0.03

−58.7 ± 0.4

1.22 ± 0.01

−68.8 ± 0.1

4.0 ± 0.2

−57.9 ± 0.9

  1. The parameters are obtained from fitting the data from three separate runs and averaging them. The errors represent ± one standard deviation.
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